Fast Neutron Radiation Induced <i>Glu-B1 </i>Deficient Lines of an Elite Bread Wheat Variety

Abstract

Five isogenic wheat lines deficient in high-molecular weight subunit (HMW-GS) proteins encoded by the B-genome were identified from a fast-neutron radiation-mutagenized population of Summit, an elite variety of bread wheat ( Triticum aestivum L.). The mutant lines differ from the wild-type progenitor by the absence of the HMW-GS Bx17 and By18 proteins. Gene specific primers designed for Bx17 and By18 genes failed to generate the diagnostic amplicons in the mutant lines. Assays using the iSelect Illumina Wheat 90K SNP arrays revealed that the induced deletions in these lines ranged from 5.58 to 127.47 cM. Isogenic wheat lines deficient in the different HMW-GS proteins will allow the determination of the contributions of these different genes to dough quality. This new germplasm will serve as genetic tools to generate healthier wheat varieties with altered dough performance for diverse end-uses.