Fast-iterative automatic reconstruction method for quantitative phase image with reduced phase perturbations in off-axis digital holographic microscopy.

Abstract

This works presents a reconstruction algorithm to recover the complex object information for an off-axis digital holographic microscope (DHM) operating in the telecentric regimen. We introduce an automatic and fast method to minimize a cost function that finds the best numerical conjugated reference beam to compensate the filtered object information, eliminating any undesired phase perturbation due to the tilt between the reference and object waves. The novelties of the proposed approach, to the best of our knowledge, are a precise estimation of the interference angle between the object and reference waves, reconstructed phase images without phase perturbations, and reduced processing time. The method has been validated using a manufactured phase target and biological samples.