Fast ibuprofen, ketoprofen and naproxen simultaneous determination in human serum for clinical toxicology by GC–FID

Abstract

ObjectivesThe aim of this study was to develop and validate a gas chromatographic method with flame ionization detection (GC–FID) for the measurement of ibuprofen, naproxen and ketoprofen for clinical toxicology purposes. Design and methods100μL of plasma was treated with methyl chloroformate and derivatized analytes were extracted with hexane. Optimal conditions of the derivatization procedure have been found using the experimental chemometric design (face-centered central composite design). The selectivity and efficiency of the procedure was confirmed by GC–MS. ResultsThe assay was linear in the concentration range of 10–400μgmL−1, with adequate accuracy and precision for GC–FID (98–106.7%, CV≤9.1%, respectively) and for GC–MS (99.3–105.5%, CV≤9.2%, respectively). ConclusionThe entire sample preparation procedure is completed within 5min and the quantitative results are available within 35min. The method was successfully applied to quantify the selected compounds in serum of patients from emergency units.