Abstract

Under optimized pulsed electric field (PEF) treatment for production of antlers calcium citrate malate and antlers collagen polypeptideby one-factor test were studied. The yield of dissoluble calcium (YDC) and protein degree of hydrolysis (DH) were used as characteristic indexes, the tests were carried out using different factors, which is as follows: the molar ratio of citric acid to malic acid (2:1-1:2), mixed acid content (0.1-1.0 mol/L), E/S (1-6 %), electric field intensity (5-30 kV/cm) and pulse number (2-12) were studied. The results indicated that the optimum conditions were E/S 4.0%, acid concentration 0.5 mol/L, electric field intensity 20 kV/cm, pulse number 8, the DH of 19.3±0.34 % and the YDC of 26±0.45 % were obtained.

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