Abstract
The performance of 5cm long narrow-bore columns packed with 2.6–2.7μm core–shell particles and a column packed with 1.7μm totally porous particles was compared in very fast gradient separations of polar neutral active pharmaceutical compounds. Peak capacities as a function of flow-rate and gradient time were measured. Peak capacities around 160–170 could be achieved within 25min with these 5cm long columns. The highest peak capacity was obtained with the Kinetex column however it was found that as the flow-rate increases, the peak capacity of the new Poroshell-120 column is getting closer to that obtained with the Kinetex column. Considering the column permeability, peak capacity per unit time and per unit pressure was also calculated. In this comparison the advantage of sub-3μm core–shell particles is more significant compared to sub-2μm totally porous particles. Moreover it was found that the very similar sized (dp=2.7μm) and structured (ρ=0.63) new Poroshell-120 and the earlier introduced Ascentis Express particles showed different efficiency. Results obtained showed that the 5cm long narrow bore columns packed with sub-3μm core–shell particles offer the chance of very fast and efficient gradient separations, thus these columns can be applied for fast screening measurements of routine pharmaceutical analysis such as cleaning validation.
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