Abstract
Methods for the development and validation for determination of iron and zinc in human serum and hair samples by graphite furnace atomic absorption spectrometry (GF AAS) were performed. Solubilization was immediate by manual agitation in both samples with a 10 mL volume sample plus deionized water. Optimum pyrolysis and atomization temperatures were obtained by pyrolysis and atomization temperature curves in both matrices. For serum samples, the best temperatures were 1400 and 2500 °C (Fe) and 700 and 1600 °C (Zn), respectively. For hair samples, the best temperatures were 1000 and 2400 °C (Fe) and 800 and 1600 °C (Zn), respectively. Permanent modifiers and zirconium presented best the results for Zn in both matrices and for Fe in serum. Permanent modifier Nb was best for hair Zn. Serum and hair values were in agreement with the certified values for analytes and certified samples. The determined values for serum Fe and hair certified samples were 1.40 ± 0.2 and 114 ± 18 mg L-1, respectively. For Zn certified reference materials (CRMs), the certified samples values were 1720 ± 32 µg L-1 and 172 ± 5 mg kg-1. Simple, accurate and precise, this method represents a cost-effective detection protocol suitable for sample analysis for diagnosis of micronutrient malnutrition.
Highlights
The importance of optimal amounts of Fe for the survival of animals, plants and microorganisms is well established and human Fe deficiency is a worldwide problem resulting from a lack of proper dietary food intake.[1]
This study investigates the methods for iron and zinc determination in serum and hair samples using graphite furnace atomic absorption spectrometry (GF AAS) after dissolution with Universol®, which presented the advantage of immediately dissolving and forming a homogeneous and indefinitely stable solution
To obtain optimum temperatures for pyrolysis and atomization of each analyte in the studied matrices and prepared by solubilization with Universol®, temperature curves were performed for pyrolysis and atomization in each matrix, using Zr as a permanent modifier for Fe and Zn in serum and Zn in hair
Summary
The importance of optimal amounts of Fe for the survival of animals, plants and microorganisms is well established and human Fe deficiency is a worldwide problem resulting from a lack of proper dietary food intake.[1]. Fast Determination of Iron and Zinc in Hair and Human Serum Samples
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