Abstract
The electrochemical behaviour of auramine O on the hanging mercury drop electrode has been investigated by cyclic and square wave voltammetry method. Reduction peak of auramine O was irreversible and adsorptive on the hanging mercury drop electrode. The optimal conditions were chosen to be Briton–Robinson buffer pH 9.0, accumulation potential −0.5 V vs. Ag/AgCl/KCl, accumulation time 60 s, pulse amplitude 250 mV·s−1, and frequency 50 Hz. At the optimum experimental conditions, the peak of the target analyte was sharp and asymmetric. The linearity of the peak current depending on the concentration ranged from 4.0 × 10−8 to 6.4 × 10−7 mol L−1. The limit of detection and limit of quantitation were 2.46 × 10−8 mol L−1 and 8.21 × 10−8 mol L−1, respectively. The recovery and relative standard deviation were 94.9% and 2.0% (n = 5). The developed method was successfully applied to determine auramine O in chicken samples with an appropriate sample preparation.
Highlights
Auramine O (AO), a diarylmethane dye, is an unauthorized food additive in Japan, the European Union, and United States due to its toxicity
We present a cathodic adsorptive stripping square-wave voltammetry method combining with Hanging mercury drop electrode (HMDE) for highly selective and sensitive determination of AO in chicken meat
To validate the results using adsorptive stripping voltammetry method, same spiked samples were sent the laboratory of Vincent certification and inspection joint stock Company. e Ultra Performance Liquid Chromatography-tandem Mass Spectrometry (UPLC-MS/MS) method was used to determine auramine O content in samples
Summary
Auramine O (AO), a diarylmethane dye, is an unauthorized food additive in Japan, the European Union, and United States due to its toxicity. Several analytical methods based on many analytical platforms have been developed for the determination of AO in food, such as spectrophotometry [7, 8], liquid chromatography [6, 9], and fluorescence [10,11,12] Their drawbacks are cost, time-consuming (due to extra procedure e.g., preconcentration, multisolvent extraction, chromatographic separation). Erefore, the amount of adsorption on the surface electrode will depend on the accumulation time For too long, it will become multilayer, impeding the analysis process. We present a cathodic adsorptive stripping square-wave voltammetry method combining with HMDE for highly selective and sensitive determination of AO in chicken meat. To adsorb AO on the surface electrode, an accumulation potential Eacc −0.5 V (vs Ag/ AgCl/KCls) was applied to the HMDE with accumulation time tacc 60 s while the solution was kept stirring. A volume of 3.0 mL of filtered solution was transferred to a 25.0 mL volumetric flask, added with 10 mL of pH 9.0 BR buffer, and made up to 25.0 mL with ddH2O
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
