Abstract
Plant growth promotion indole-3-acetic acid (IAA) is the most abundant natural auxin that plays diverse roles in plant growth, development and plant immunity. Perturbing auxin homeostasis appears to be a common virulence mechanism, as many pathogens can synthesize auxin-like molecules. In other hand, the addition of plant growth promotion rhizobacteria (PGPR) that are able to produce auxins promotes plant growth and provides protection against pathogens. Techniques as high performance liquid chromatography (HPLC) and gas chromatography (GC) are used to quantify auxins produced by microorganism and plants at high precision and sensitivity, even though those techniques are expensive and require a big number of solvents. For these reasons, the aim of the present study was to develop a fast microplate technique for auxin detection, in Bacillus subtilis strains using salkowski reagent. For auxin quantification, calibration curves were done with alcohol, landy medium and water and the R2 were calculated. The microplate techniques were able to quantify auxin production by B. subtillis stains.
Highlights
Plant hormones have pivotal roles in the regulation of plant growth, development, and reproduction [1]
Techniques as high performance liquid chromatography (HPLC) and gas chromatography (GC) are used to quantify auxins produced by microorganism and plants at high precision and sensitivity, even though those techniques are expensive and require a big number of solvents
Recent studies indicate that hormones such as abscisic acid (ABA), auxin, gibberellic acid (GA), cytokinin (CK), brassinosteroids (BR) and peptide hormones are implicated in plant defense signaling pathways [2]
Summary
Plant hormones have pivotal roles in the regulation of plant growth, development, and reproduction [1] They emerged as cellular signal molecules with key functions in the regulation of immune responses to microbial pathogens, insect herbivores, and beneficial microbes [1]. Perturbing auxin homeostasis appears to be a common virulence mechanism, as many pathogens can synthesize auxin-like molecules [5] This way, the addition of PRGR producers of auxins promotes plant growth and provides protection against pathogens. For auxin and indoles detection, techniques are used such as high performance liquid chromatography (HPLC) [3], thin layer chromatography (TLC) [11] and spectrophotometric techniques with salkowski reagent [12] Even though these techniques are expensive because of the use of columns and solvents (HPLC) or are not practical for processing big quantity of samples at the same time (TLC). The microplate techniques developed were used to quantify auxins in Bacillus subtilis fermentation broths proved previously as auxin producers
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