Fast color fourier Ptychographic microscopic imaging technology with fusion color correction

Abstract

In biomedicine, rapid and accurate acquisition of color pathology slides is of paramount importance for diagnostic analyses. Conventional microscopic full-color imaging cannot provide the physician with enough effective information at one time due to the "law of proportionality of objective lenses." Fourier ptychographic microscopy (FPM) has emerged as a solution for high-throughput digital pathology. It can simultaneously achieve a large field of view and high-resolution (HR) imaging. However, achieving color FPM typically requires capturing images separately for each of the red, green, and blue channels for reconstruction, resulting in a significant increase in the time cost. Existing improvement strategies require long coloring times, and have difficulty ensuring standardization between the reconstructed and original image colors owing to the influence of optical system stability and algorithm errors. In this study, a fast-color FPM imaging technology with fusion color correction (CCFPM) is proposed. It achieves high-precision color FPM imaging by separately capturing single low-resolution images for the red and blue channels, which are used to color the reconstructed green-channel HR grayscale image. The reconstructed HR color image is then processed using a color correction algorithm. Simulation and experimental analyses demonstrate that this method compresses the coloring algorithm's computation time to less than 1 s, reducing both the acquisition and reconstruction times by 66.7 % compared with the traditional R + G + B channel approach, achieving a significant improvement in speed. Meanwhile, it also achieves a breakthrough in algorithm speed compared with other fast color FPM solutions, compressing the coloring time to a few hundredths or even a few thousandths of the existing algorithms, and ensures the accuracy of the reconstructed image, reducing the RMSE by 50 % compared with the existing algorithms. Regarding color consistency, CCFPM can mitigate the chromatic aberration caused by fluctuations in optical system stability, maintaining consistency between the reconstructed and original image colors, thus achieving standardization of image color appearance. Therefore, the proposed method is promising for further advancements in the application of FPM in digital pathology.