Abstract
The exposure of a cold-adapted rat for 15 min at 4°C results in the appearance of measurable oxidation of added NADH via the external amytal- and antimycin-A- resistant pathway in liver mitochondria. This effect increases if the animal has been treated with thyroid gland preparation for 4–5 days. In vitro, the addition of Mg 2+ or increase in the tonicity of incubation mixture suppresses external NADH oxidation. Addition of 70 mg · ml −1 serum albumin or 70 mg · ml −1 polyvinylpyrrolidone in the presence of Mg 2+ and addition of 20 μM palmitic or 20 μM oleic acids induces to some extent external NADH oxidation in the mitochondria of control (nontreated) and of cold-adapted, thyroidgland-preparation-treated rats. It is concluded that hyperhyroid rats can be used as a model of cold-induced initiation of the external pathway of NADH oxidation. A relation between the increase of exogenous NADH oxidation and phospolipase A 2 activity in liver mitochondria is discussed.
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