Abstract
Understanding the three-dimensional (3D) landscape of the DNA damage response (DDR) is crucial to unveiling genome maintenance mechanisms. Furthermore, accurate kinetic analysis of the DDR mechanisms grants better comprehension of the cellular response from lethal DNA lesions. To achieve the necessary temporal resolution and induce precise, controllable DSBs, we recently developed a novel technology, termed very fast CRISPR (vfCRISPR). This technique enables controllable, site-specific double-strand DNA break (DSB) generation in living cells within only a few seconds of photoactivation of bound Cas9.
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