Abstract
Cytochrome P-450 isoenzyme are detected very rapidly (in <5 s) by their catalysis of the luminol-cumene hydroperoxide chemiluminescent reaction. The detection limit is 0.01 μl of microsomes. The system is applied to detect the isoenzymes from rat liver microsomes after separation of column chromatography on amino-octyl agarose and DEAE-Biogel A columns.
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