Abstract
Fast atom bombardment mass spectrometry (FAB MS) has been used to measure the relative abundances of zinc stable isotopes in biological samples. Modification of the standard FAB sample target, moderate MS resolution, and ion exchange column chromatography have been used to eliminate interfering ions at the nominal masses of zinc isotopes. Precision of 1.0% coefficient of variation was obtained on fecal samples at a 1% level of isotopically enriched (polyisotopic) preparation. The detection limit has been established as 0.05% of the polyisotopic preparation in the sample (3sigma). In addition, this work corroborated the previous suggestion that isotope fractionation occurs with FAB MS of metals. After correction for this fractionation, a plot of measured vs. calculated isotope ratios exhibited a slope of 1.012 and an r of 0.998. With the techniques developed, isotope studies have been conducted on adult volunteers, and these demonstrated a 34-36% excretion of the administered zinc isotope preparation.
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