Abstract
Single-molecule FRET (smFRET) microscopy is a powerful tool for monitoring the conformational diversity and structural dynamics of individual biomolecules. However, the full data analysis of single molecule FRET movies is often a complex and time-consuming task. Here, we present a fast, robust and user-friendly data analysis platform for smFRET microscopy of immobilized molecules. The presented toolkit can be operated without any prior programming skills and allows different aspects of smFRET data to be analyzed in an explorative and extensible framework. We illustrate its capabilities using data of different double stranded DNA and G-quadruplex structures.
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