Abstract
The aim of this study was to develop a method for identification and quantification of diazepam and its major metabolite, desmethyldiazepam in plasma samples in psychiatric patients. Liquid - liquid extraction (LLE) procedure was applied in order to simplify the pre-analytical phase, followed by gas chromatography � mass spectrometry (GC-MS) detection and gas chromatography � flame ionization (GC-FID) quantitation.
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