Abstract
A new approach for biogenic amines (BA) determination in fermented beverages was developed coupling ultrafast dansylation conditions with ultra-high-performance liquid chromatography (UHPLC) method. Derivatization conditions for 8 amines analysis were optimized using D-optimal and central composite designs reducing in 88% the reaction time. Dansylated amines were separated on C18 column using a mobile phase composed of ammonium formate buffer and acetonitrile. Detection was performed by UV and fluorescence. Migration from conventional HPLC to UHPLC allowed a 60% reduction in analysis. The developed method was validated following ICH recommendations. Calibration data fitted a linear regression model with R2 were values highest to 0.984. Repeatability and intermediate precision in matrix showed relative standard deviation (RSD) values lower than 4.86 and 11.53%, respectively. Detection and quantification limits ranged from 0.03 to 0.18 and from 0.20 to 0.59 mg mg L−1. BA content in beer and wine samples were 10.53 to 73.17 mg L−1.
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