Abstract
The speciation analysis of As in urine samples can provide important information for epidemiological studies. Considering that these studies involve hundreds or thousands of samples, a fast and reliable method using a simple LC system with short-length mixed bed ion exchange chromatographic column coupled to ICP-MS for As speciation in human urine samples was developed in this work. Separation of AB+TMAO, DMA, AC, MMA and AsIII+AsV was accomplished within 5min with good resolution when ammonium carbonate solutions were used as mobile phases and H2O2 was added to samples to quantitatively convert AsIII–AsV. Repeatability studies yielded RSD values from 2.0% to 4.8% for all species evaluated. Limits of detection (LOD) for As species ranged from 0.003 to 0.051ngg−1. Application of the method to human urine samples from a non-contaminated area showed that the sum of species measured corresponded to 62–125% of the total As in the sample. The recovery values for these species in urine SRM 2669 were in the range of 89–112% and demonstrated the suitability of the proposed method for epidemiological studies.
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