Abstract
The application of transmission electron microscopy (TEM) for the research on biological and inorganic structures is restricted by the radiation sensitivity of the specimens and the deviations from the optimal setting of the TEM parameters. The three most important optical parameters that require extensive fine tuning are the defocus, astigmatism and beam tilt misalignment. Autotuning, i.e. automatic optimization of these three parameters, can help to optimize microscope settings using a minimum amount of electron irradiation.Until recently, the practical applications of the autotuning methods proposed in literature were limited due to insufficient computational power and the lack of commercially available computer interfaces to TEM. These instrumental limitations can now be overcome be a fully remote controllable TEM, a highly sensitive video camera system, and a fast image processing system.
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More From: Proceedings, annual meeting, Electron Microscopy Society of America
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