Farmed edible bird’s nest in malaysia: proximate analysis, safety profile, epidermal growth factor content and effect on cancer cells

Abstract

Event Abstract Back to Event Farmed edible bird’s nest in malaysia: proximate analysis, safety profile, epidermal growth factor content and effect on cancer cells Sin N. Tan1*, Careena Shobana1, Sani Dahiru1, Chee W. Lim1, Ideris Aini1, Stanslas Johnson1 and Thiam S. Christopher Lim1 1 Putra Malaysia University, Malaysia Background Edible bird’s nest (EBN), a solidified swiftlet's saliva, is the most expensive animal products consumed by humans worldwide. EBN exhibits multiple medicinal properties and contains many bioactive compounds such as sialic acid, amino acids, fatty acids, minerals and antioxidants. In recent years, many safety issues which included high nitrate and nitrite contents, presence of heavy metal, adulteration, fungal infection and cancer cell stimulation were found to be associated with EBN. Hence, this study was carried out to determine the proximate analysis, safety profile, epidermal growth factor content and its effect on cancer cells of Malaysia EBN. Methods Proximate analysis and safety profile were performed by using official AOCA methods according to the Malaysia Standard MS 2509:2012. Coupled Plasma Optical Emission Spectrophotometer was adopted for heavy metals analysis. Enzyme-linked immunosorbent assay was used for quantification of EGF content. Microculture tetrazolium assay was utilised for assessment of growth stimulation by different concentration of human epidermal growth factor (hEGF) in comparison to EBN in 4 different cancer cell lines: MCF-7 (human breast adenocarcinoma cells), Caco-2 (human epithelial colorectal adenocarcinoma cells), HCT116 (human colorectal carcinoma), and A549 (human alveolar adenocarcinoma cells) for 24 hours, 48 hours, and 72 hours. Results Our results showed a consistency of high protein (53.03% - 56.37%) and carbohydrate (27.97%-31.68%) with acceptable level of moisture (10.80-14.04%) and ash (2.22%-3.38%). Besides, a good safety profile was obtained with low nitrite and nitrate level, undetectable heavy metals and no significant growth for pathogenic microorganism. The present study found above tolerance level of yeast and moulds in EBN. Haze contaminated EBN exhibited no significant differences in terms of nutritional, heavy metal and microbiology profiles. EFG (30.7pg/mL and 74.5pg/mL) were detected in crude EBN01 and EBN02, respectively but not in all digested EBN samples and even post 10 times concentrated EBN. Cancer cell growths were significantly increased after treatment with hEGF. No significant growth was observed after treatment with EBN. Conclusion EBN in Malaysia is free from heavy metals, within tolerance level of nitrate and nitrite, and also microbiology profile. Furthermore, in vitro study indicated that EBN is not associated with cancer cell stimulation. Keywords: Cancer, Epidermal Growth Factor, Safety, nutritional, Edible bird's nest Conference: International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18) “Seizing Opportunities and Addressing Challenges of Precision Medicine”, Putrajaya, Malaysia, 3 Dec - 5 Feb, 2019. Presentation Type: Poster Presentation Topic: Cancer Citation: Tan SN, Shobana C, Dahiru S, Lim CW, Aini I, Johnson S and Christopher Lim TS (2019). Farmed edible bird’s nest in malaysia: proximate analysis, safety profile, epidermal growth factor content and effect on cancer cells. Front. Pharmacol. Conference Abstract: International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18) “Seizing Opportunities and Addressing Challenges of Precision Medicine”. doi: 10.3389/conf.fphar.2018.63.00130 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 30 Sep 2018; Published Online: 17 Jan 2019. * Correspondence: Dr. Sin N Tan, Putra Malaysia University, Seri Kembangan, Malaysia, christine0507@live.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Sin N Tan Careena Shobana Sani Dahiru Chee W Lim Ideris Aini Stanslas Johnson Thiam S Christopher Lim Google Sin N Tan Careena Shobana Sani Dahiru Chee W Lim Ideris Aini Stanslas Johnson Thiam S Christopher Lim Google Scholar Sin N Tan Careena Shobana Sani Dahiru Chee W Lim Ideris Aini Stanslas Johnson Thiam S Christopher Lim PubMed Sin N Tan Careena Shobana Sani Dahiru Chee W Lim Ideris Aini Stanslas Johnson Thiam S Christopher Lim Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.