Farklı Tekniklerle Ekstrakte Edilen Propolisin Polifenol İçerikleri Ve Antioksidan Aktivitelerinin Değerlendirilmesi

Abstract

Propolis is classified as an opotherapeutic medicine due to the botanical origin of the resins. The chemical composition of propolis is greatly influenced by the honeybee species, botanical source and extraction techniques. Within this frame, we compared the same propolis’ polyphenol contents and antioxidant activities prepared with different techniques. Four types of extracts were prepared. The first type was prepared classically by ethyl alcohol (POH). The second and third types were extracted by sterile distilled water kept as both sterilised (PS) and non-sterilized (PN). The fourth one was prepared with full vacuumed and dried propolis with honey (PH). The antioxidant activity of extracts was evaluated with DPPH radical scavenging, ABTS radical cation scavenging, Cupric ion reducing antioxidant capacity. Also total phenolic and flavonoid content of extracts were investigated. POH extract showed significantly high content of total phenol and flavonoids which followed by PN, PS and PH. POH showed approximately two times higher activity on DPPH radical (IC50=4,39μg/mL) compared with quercetin as references. The highest activity on DPPH is shown by POH with 4,39 μg/mL of IC50 value which was followed by aqueous extracts 18,08. The lowest activity was shown by PS with 4,39 μg/mL of IC50 value. The highest scavenging activity against ABTS radical cation was shown by POH (73,37 mg TE/g extract) and the lowest activity was shown by PS (34,21 mg TE/g extract). According to the results, the new aqueous extraction technique is promising with relatively high polyphenol contents and antioxidant activities. Also honey with propolis can be an alternative product, although it has relatively lower values of antioxidant activity.