Abstract
The aim of this research was the isolation of thermophilic Bacillus sp. from different sources and grouping by 16S-ITS rDNA RFLP. Seventy-four thermophilic strains were isolated from various environmental samples. Since fifteen isolated strains were Gram (+), endospore forming rods, they were identified as Bacillus sp. These strains were screened for the existence of five extracellular enzyme activities. These were, lipases, amylases, proteases, xylanases, cellulases. 16S-ITS rDNA RFLP profiles were produced by using two restriction endonucleases Taq I and Hae III. The isolated strains were clustered into eigth groups by Taq I restriction profiles of 16S-ITS rDNA while seven groups were obtained by Hae III digestion profiles.
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