Farklı Çevresel Koşullara Uyumlu Bazı Sazan Türlerinde Hemoglobin Proteini Farklılıklarının Elektroforetik Tekniklerle Araştırılması

Abstract

Six different temperate zone species of Cyprinidae family members, which are adapted to different harsh environmental conditions, were analyzed versus cold-acclimated Salmonid. Hemoglobin diversity in the studied species was investigated with a modified form of isoelectric pH: 3.5-10 ampholite system. 6 isohemoglobin bands were observed for cold-acclimated Oncorhynchus mykiss 8 isohemoglobin were observed for Chalcalburnus tarichi 9 for Cyprinion macrostomus and Sarkisla (Sivas) Cyprinus carpio, 11 Garra rufa and finally 12 for Todurge (Sivas) Cyprinus carpio. Cathodic/anodic ratios were found equal to 1for control and the fish adapted to available conditions. However, these ratios were found below 1 when environmental conditions were changed for fish, which are adapted to these poorly oxygenated conditions. Different environmentally adapted fish species are representing various isohemoglobins bands pattern. The species used as a control group in this study shown the lower isohemoglobin bands form. On the other hand, different forms of temperate climate Cyprinidae family member are produced isohemoglobins bands pattern, and the number of these band were higher than control group, depending on different adaptation conditions. The majority of these protein bands were anadic and it can be said that fish are producing anodic hemoglobin protein, when environmental conditions was changed to poorly oxygenated milieu.