Faraday cage-type aptasensor for dual-mode detection of Vibrio parahaemolyticus.

Abstract

AFaraday cage-type aptasensor has been developedfor dual-mode detection of a common bacterial pathogen Vibrio parahaemolyticus (VP) by electrochemiluminescence (ECL) and differential pulse voltammetry (DPV), using a multi-functionalized material Pb2+-Ru-MOF@Apt2 as signal unit. Therecognition aptamer Apt2 recognizes VP; specifically, ruthenium-based metal organic framework (Ru-MOF) and lead ions (Pb2+) embedded produce anECL signal at a working potential from 0 to 1.5V and DPV signal from - 0.3 to - 0.8V vs. Ag/AgCl. SinceRu-MOF is a two-dimensional conductive material signal unit overlapped onto the electrode surface to form a Faraday cage-type aptasensor. Thus, electrons could be easily exchanged between electrode and signal tags without being hindered by micron-size VP, resulting in a high detection sensitivity with a detectionlimit of 1.7CFUmL-1. In addition, dual-mode detection was achieved, improving the accuracy and reliability of rapid field detection. Stability and selectivity were alsosatisfactory. The tests of real samples indicate that this Faraday cage-type aptasensor is suited forrapid detection of VP and analog pathogens and shows great potential in food safety. Graphical abstract.