Abstract
AbstractFamily shuffling, which generates chimeric progeny genes by recombining a set of naturally occurring homologous genes, is an extremely powerful approach for in vitro protein evolution. In comparison with other in vitro protein evolution methods, family shuffling has the advantage of sampling a larger portion of the sequence space that has been proven functionally rich by nature. So far, the most widely used technique to carry out family shuffling is DNA shuffling (1–5). However, one significant drawback associated with this method is the low frequency of chimeric genes (recombined gene products) in the shuffled library (6), which may be largely owing to the annealing of DNA fragments derived from the same parental genes (homo-duplex formation) whose probability is much higher than that of hetero-duplex formation. This is true even when the sequence homologies among parental genes are higher than 80% (7).KeywordsChimeric GeneElongation TimeLambda ExonucleaseShuffling MethodRecombine Gene ProductThese keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
