False aminoglycoside resistance in Enterobacterales and non-fermenters by an automated testing system: a descriptive study.

Peter M C Klein Klouwenberg ,M De Graaf,Sander Dinant ,Wouter Van Den Bijllaardt ,Damian C Melles∗ ,Maarten Scholing ,A J Van Griethuysen ,J De Vries ,Anouk E Muller ,W J Van Den Reek ,Martha T Van Der Beek ,L.g.m Bode ,Alewijn Ott ,L J Bakker ,J Sinnige ,E.i.g.b De Brauwer ,G Blaauw ,Marcus Ho Yin Wong ,A Jansz ,Wieke Altorf-Van Der Kuil ,Nicole H M Renders ,Ben Wit ,J W Dorigo-Zetsma ,A L M Vlek ,Sabine C De Greeff ,Michiel Haeseker ,Erik Bathoorn ,E.m Kraan ,Jos Monen ,R Hertroys ,M Van Rijn ,J.m Da Silva ,Ellen De Jong ,Ed J Kuijper ,Niek Marchal ,Michael Hall ,B Maraha ,E Schaftenaar ,S.m Bierman ,Suzan P Van Mens ,Michelle Hendriks ,Cornelia C H Wielders ,Mariëtte Lokate ,Annelot F Schoffelen ,A Alzubaidy ,David S Y Ong ,Caroline Schneeberger-Van Der Linden ,S R Groenendijk ,J W T Cohen Stuart ,Karola Waar ,Bram M W Diederen ,Patrick Sturm ,Robbert G Bentvelsen ,Jan Polman ,O Pontesilli ,Annet Troelstra ,M J C A Van Trijp ,B.b Wintermans ,A G M Buiting ,Karin Van Dijk ,D W Van Dam ,Marrigje H Nabuurs-Franssen ,Ianthe Maat ,Sacha D Kuil ,Daan W Notermans ,R E Zoetigheid ,Maurits P A Van Meer ,J H Machiels

doi:10.1128/spectrum.03093-23

Abstract

Antimicrobial sensitivity data are important to guide antimicrobial therapy. In microbiological laboratories, routine sensitivity measurements are typically performed with automated testing systems such as VITEK2 and Phoenix. Using data from the Dutch national surveillance system for antimicrobial resistance over a 6-year period, we found that the measured minimum inhibitory concentrations for aminoglycosides in Enterobacterales and non-fermenters were too high for the Phoenix system. In addition, we observed a yearly increase in resistance for several species measured by Phoenix. These findings might have consequences for clinical treatment of patients with sepsis.

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