Abstract

1. 1. The plains killifish Fundulus kansae regulated plasma osmotic concentrations within narrow limits between fresh water and 66‰ sea water. 2. 2. Freshwater-held fish survived transfer to 38.5‰ sea water. 3. 3. With gradually increasing salinity, fish could survive in 99‰ sea water. 4. 4. Freshwater-held fish tolerated plasma osmotic concentrations of about 640 mOsm after transfer to sea water. 5. 5. Fish acclimated to hypersaline sea water (99‰) tolerated plasma osmotic concentrations of about 630 mOsm. 6. 6. We conclude that acclimation to sea water does not significantly alter cellular tolerance to high osmotic concentrations.

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