Abstract
Eleven-day-old rat maxillary first molar explants were removed by microdissection and incubated in vitro to determine the direct effects of parathyroid hormone (PTH), calcitonin (CT), 1,25(OH)2 D3 , 24.25(OH) 2 D3, and a combination of PTH and vitamin D 3 metabolites on calcium uptake in the mineralizing enamel of the explants. None of the agents had a statistically significant effect. These results are in contrast to those observed on explants from six-day-old rats, where PTH + 24,25(OH)2 D3 caused a significant increase in net calcium transport. The findings are consistent with the hypothesis that the transcellular transport of calcium through the secretory stage and the maturation stage ameloblasts occurs by different mechanisms.
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