Abstract
We previously reported that young (2-to 3-day-old) embryonic chick hearts organ cultured in vitro for 1–2 weeks in glass vessels do not gain the TTX-sensitive fast Na+ channels, as they normally would during in situ development. Instead, they retain their high density of TTX-insensitive slow Na+ channels. In the present study, 3-day hearts were organ cultured on the chorioallantoic membrane of host 6-to 7-day-old eggs to make the culture condition closer to that in situ. Hearts cultured by this method for 6–8 days also did not gain fast Na+ channels or lose any of their slow Na+ channels. Hence, something in the in situ condition is necessary for the induction of the changes in membrane properties.
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