Failure of cytokinesis and 2n gamete formation in Brazilian accessions of Paspalum

Abstract

Cytological analysis on 112 Brazilian accessions of Paspalum showed that 52 were affected by failure of first or second cytokinesis during microsporogenesis. When the failure of cytokinesis occurred only in the first division and in the second division the cytokinesis was normal, a dyad of microspores was formed. In most of them the two cells remained binucleate, but in some a restitutional nucleus was observed. When the first division was normal and the failure of cytokinesis occurred only in the second division, dyads and triads were observed. A restitutional nucleus in one or both cells of the dyad, or in the binucleate cell of the triad was also observed. The percentage of dyads and triads varied among accessions, reaching 15.75 of the sporads in some. The absence of cytokinesis indicates the possibility that the widespread occurrence of polyploidy in the genus Paspalum originates from 2n pollen grains.