Abstract
This study presents a molecular inhibitory mechanism by Fas-associated factor 1 (FAF1) on IκB kinase (IKK) activation, where divergent NF-κB-activating stimuli converge. FAF1 interacts with IKKβ in response to proinflammatory stimuli (such as tumor necrosis factor-α, interleukin-1β, and lipopolysaccharide) and suppresses IKK activation. Interaction of the leucine-zipper domain of IKKβ with FAF1 affected the IKK heterocomplex (IKKα/β) and homocomplex (IKKα/α, IKKβ/β) formations and attenuated IKKγ recruitment to IKKβ. Overexpression of FAF1 reduced the level of IKKβ activity, whereas FAF1 depletion increased the activity. These results indicate that FAF1 inhibits IKK activation and its downstream signaling by interrupting the IKK complex assembly through physical interaction with IKKβ. Taken together, FAF1 robustly suppresses NF-κB activation through the inhibition of IKK activation in combination with previously reported cytoplasmic retention of NF-κB p65 (Park, M. Y., Jang, H. D., Lee, S. Y., Lee, K. J., and Kim, E. (2004) J. Biol. Chem. 279, 2544–2549). Such redundant suppression would prevent inadvertent activation of the NF-κB pathway.
Highlights
Fas-associated factor 1 (FAF1)3 is evolutionarily conserved from flies to mammals [1,2,3,4] and is involved in various key biological processes
Overexpression of FAF1 reduced the level of IKK activity, whereas FAF1 depletion increased the activity. These results indicate that FAF1 inhibits IKK activation and its downstream signaling by interrupting the IKK complex assembly through physical interaction with IKK
This study reveals a novel function of FAF1: as an endogenous suppressor of IKK activation
Summary
Fas-associated factor 1 (FAF1)3 is evolutionarily conserved from flies to mammals [1,2,3,4] and is involved in various key biological processes. FAF1 interacts with IKK in response to proinflammatory stimuli (such as tumor necrosis factor-␣, interleukin-1, and lipopolysaccharide) and suppresses IKK activation. These results indicate that FAF1 inhibits IKK activation and its downstream signaling by interrupting the IKK complex assembly through physical interaction with IKK. FAF1 disrupts IKK complex assembly through physical interaction with the LZ domain of IKK.
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