Abstract
Electroenzymatic reduction of NAD + to NADH for subsequent use in enzymatic synthesis has been carried out at carbon electrodes bearing lipoamide dehydrogenase (LiDH) immobilized under a Nafion R film. The self-mediated electron transfer was made possible by an excess of flavin adenine dinucleotide (FAD) entrapped together with LiDH. Results were compared to those obtained with a similar electrode containing both LiDH and a polymeric form of FAD (pFAD) prepared by anodic polymerization of FAD. Electroenzymatic regeneration of NAD + to NADH coupled to enzymatic synthesis of l-lactate has been done using FAD or a polymeric FAD (prepared by anodic polymerization) as mediators.
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