Abstract
Forty-two bacterial strains that produce calcium 2,5-diketo-d-gluconate from 30% (w/v) Dglucose with yields of more than 70 mol% were isolated from various soil and fruit samples. They were small rod-shaped, gram-negative, non-spore-forming and facultatively anaerobic bacteria, and could be classified taxonomically into three groups. Representative strains (SHS 2003, ATCC 31623; SHS 2006, ATCC 31626; SHS 2008, ATCC 31628) of the latter were identified as members of the genus Erwinia. The DNA base ratios of these strains ranged from 46.3 to 48.7 mol% guanine plus cytosine.The results of experiments using whole cells and cell extracts of these three strains suggested that d-glucose is oxidized to 2,5-diketo-d-gluconate via d-gluconate and 2-keto-d-gluconate. No 5-keto-d-gluconate was detected in the culture broth throughout their cultivation. Most activities of enzyme(s) responsible for the oxidation of d-glucose to 2,5-diketo-d-gluconate were found in the particulate fractions of these strains.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
