Abstract
BackgroundThe understanding of the roles of gut bacteria in the fitness and vectorial capacity of mosquitoes that transmit malaria, is improving; however, the factors shaping the composition and structure of such bacterial communities remain elusive. In this study, a high-throughput 16S rRNA gene sequencing was conducted to understand the effect of developmental stage, feeding status, species, and geography on the composition of the gut bacterial microbiota of two main Colombian malaria vectors, Anopheles nuneztovari and Anopheles darlingi.ResultsThe results revealed that mosquito developmental stage, followed by geographical location, are more important determinants of the gut bacterial composition than mosquito species or adult feeding status. Further, they showed that mosquito gut is a major filter for environmental bacteria colonization.ConclusionsThe sampling design and analytical approach of this study allowed to untangle the influence of factors that are simultaneously shaping the microbiota composition of two Latin-American malaria vectors, essential aspect for the design of vector biocontrol strategies.
Highlights
The understanding of the roles of gut bacteria in the fitness and vectorial capacity of mosquitoes that transmit malaria, is improving; the factors shaping the composition and structure of such bacterial communities remain elusive
Anopheles species composition and abundances A total of 239 Anopheles specimens were collected in two municipalities of two important malaria-endemic regions of Colombia: Istmina (IST), located in the Pacific coast (PAC), western Colombia and El Bagre (BAG) in the Urabá-Bajo Cauca-Alto Sinú (UCS), northwest Colombia
The relative abundance of the third class, Betaproteobacteria, showed its maximal occurrence in larval samples. These results indicate that most of the aquatic bacterial colonizers persist transstadially from larvae to adults (WLA), the relative abundance of bacterial classes clearly change in the mosquito gut during the developmental stages
Summary
The understanding of the roles of gut bacteria in the fitness and vectorial capacity of mosquitoes that transmit malaria, is improving; the factors shaping the composition and structure of such bacterial communities remain elusive. A high-throughput 16S rRNA gene sequencing was conducted to understand the effect of developmental stage, feeding status, species, and geography on the composition of the gut bacterial microbiota of two main Colombian malaria vectors, Anopheles nuneztovari and Anopheles darlingi. Thereby, the aim of this study was to analyze simultaneously the effect of four factors upon the gut bacterial communities of two Colombian field-collected malaria vectors, Anopheles darlingi and Anopheles nuneztovari. To our knowledge, this is the first study that examines the gut microbiota of these two main Latin American malaria vectors using a high-throughput sequencing approach
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
