Abstract
Lacassagne found several years ago that the rabbit-papilloma virus is notably resistant to X-rays,1 and Syverton has more recently reported that a dose of 14,000,000 r was required to abolish the infectivity of a cell-free suspension of the papilloma virus2 though 1,000,000 r or less sufficed to inactivate certain other viruses and bacteria. In experiments undertaken lately for other purposes we have noted that the amount of irradiation required to inactivate the papilloma-virus is influenced by the virus concentration, as also by extraneous material present along with it in the irradiated suspensions. Berkefeld filtrates, which contained the virus in large amount, were rendered completely non-infectious upon exposure to 2 to 4 million r of irradiation, while only 400,000 to 800,000 r was required when the virus had been partially purified by repeated differential centrifugations.Virus was obtained by grinding infectious cottontail rabbit papil-loma-tissue and extracting it in 10 or 20 volumes of isotonic...
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