Abstract
The effect of various experimental parameters upon the frequency of callus formation from cultured anthers of Oryza sativa has been investigated. Although certain medium components were found to be critical to callus formation, the concentration of these components had little effect upon the frequency of callus formation. The degree to which the callus formation frequency was influenced by cold pretreatment of the flowers was variable. Even though plants were grown under uniform conditions and flowers containing pollen in the microspore stage of development were selected for dissection, the frequency of callus formation varied nonrandomly between flowers. In experiments with populations of flowers in a physiological and developmental state favorable to callus formation 35% of plated anthers produced callus and at least 60% of these calluses gave rise to green plants.
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