Abstract
Somatic embryos derived from staminate inflorescence tissues of three mature sweetgum (Liquidambar styraciflua) trees were tested for germination and conversion frequencies and early growth variables following pregermination cold treatments. Individual mature embryos were selected from repetitively embryogenic cultures maintained on Woody Plant Medium (WPM) with 1 mg l−1 naphthaleneacetic acid and cultured on basal WPM at 10 8C for 0, 4 or 8 weeks prior to being transferred to WPM germination medium in the light. After 4 weeks, germinated embryos were planted in potting mix in an acclimatization chamber, grown for an additional 8 weeks and evaluated for conversion and growth. Conversion frequency, which ranged up to 80%, was affected by ortet and clonal line, while the number of first-order lateral roots was affected by ortet, clonal line and cold treatment, with 8 weeks of cold promoting the highest number.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.