Abstract
Somatic embryos derived from staminate inflorescence tissues of three mature sweetgum (Liquidambar styraciflua) trees were tested for germination and conversion frequencies and early growth variables following pregermination cold treatments. Individual mature embryos were selected from repetitively embryogenic cultures maintained on Woody Plant Medium (WPM) with 1 mg l−1 naphthaleneacetic acid and cultured on basal WPM at 10 8C for 0, 4 or 8 weeks prior to being transferred to WPM germination medium in the light. After 4 weeks, germinated embryos were planted in potting mix in an acclimatization chamber, grown for an additional 8 weeks and evaluated for conversion and growth. Conversion frequency, which ranged up to 80%, was affected by ortet and clonal line, while the number of first-order lateral roots was affected by ortet, clonal line and cold treatment, with 8 weeks of cold promoting the highest number.
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