Factors influencing hematopoietic spleen colony formation in irradiated mice. V. Effect of foreign plasma upon colony forming cell kinetics.

Abstract

AbstractForeign plasma injection induces a profound and somewhat complex change in the size and location of the colony forming unit (CFU) cell compartment. Injection of foreign plasma before irradiation induces an increase in CFU cells as judged by endogenous colonies as well as by a modification of the endogenous method which excludes spleen colony formation from in situ spleen cells. However, the enlargement does not take place in the most populous CFU cell areas, the spleen and marrow. The concentration and/or total number of CFU cells in spleen and marrow was not increased by plasma injection whether judged by the number of transplantable cells or by the number of migrating endogenous cells.These studies emphasize the complexity of this cellular system and suggest that the use of but one type of stem cell assay may yield results which do not reflect changes within the total compartment. Evidence for cell damage in vitro as a factor influencing results in studies involving transplantation was searched for but was not forthcoming.