Abstract
In apple maggot fly, Rhagoletis pomonella (Walsh), using females that had mated twice, we investigated the occurrence of sperm predominance by using electrophoresis to analyze four enzymes (allozymes) of parents and offspring. A maximum likelihood method was used to estimate paternity when males did not differ unambiguously at any of the four loci. We found predominance of second male sperm in most cases; 79–93% of offspring were fathered by the second male. The degree of sperm predominance was not significantly affected by male mating treatment, number of days of female egglaying, or method of paternity determination (estimated or exact).
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