Abstract
Abstract 1. 1. Gas-liquid radiochromatography has been used to determine the chain lengths of fatty acids synthesised by extracts of lactating-rabbit mammary glands. 2. 2. The particle-free supernatant fraction synthesised mainly short-chain fatty acids. Addition of “microsomal” protein (and to a lesser extent mitochondrial protein) increased the overall rate of synthesis, particularly that of long-chain fatty acids. 3. 3. The effects of time of incubation, ATP, CoA, bivalent cations, reduced pyridine nucleotides, HCO3- and avidin on the chain lengths of the fatty acids synthesised has been investigated. Results indicate that butyrate can be synthesised by two separate mechanisms. 4. 4. Synthesis was stimulated by a number of dicarboxylic acids, malonate and succinate being the most effective. Citrate caused a greater stimulation, which was competitive with that of malonate. Stimulation by citrate and malonate was greatest for synthesis of longer chain-length fatty acids (C14:0 and C16:0) using the microsomal plus particle-free supernatant fraction, but greatest for C8:0, C10:0 and C10:0 using the particle-free supernatant fraction. 5. 5. Rates of incorporation of acetate, citrate and malonate into individual fatty acids have been compared. 6. 6. The concentration of malonyl-CoA in incubation systems has been found to directly influence the chain lengths of fatty acids synthesised.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: BBA - Specialised Section On Lipids and Related Subjects
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
