Abstract
Production of adventitious buds in culture of cotyledon explants derived from young seedlings of Douglas fir ( Pseudotsuga menziesii (Mirb.) Franco) required both cytokinin and auxin. A pronounced influence on morphogenetic responses by variations in physiological condition or genetic complement was observed using the natural auxins, IAA and IBA. However, this influence was nearly eliminated when appropriate concentrations of synthetic auxin, NAA and/or 2,4-D, were used. BAP was more effective in stimulation of organ formation than 2iP or kinetin. A high frequency of organ differentiation was obtained with 5 μM BAP plus either NAA or 2,4-D at 0.5–50 nM.
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