Factors Associated with Occurrence and Reversibility of Connective Tissue Attachment Loss

Abstract

Marginal periodontitis appears to be site-specific, demonstrates rapid bursts of connective tissue attachment destruction, and may, at times, repair spontaneously. The present study was undertaken to investigate associations between bacterial populations and periodontal destruction parameters within the first 14 days after induction of experimental periodontitis in animals receiving metronidazole. Metronidazole was administered orally to four squirrel monkeys for 17 days at a daily dose of 100 mg/kg body weight. After three days (baseline), marginal periodontitis was induced by tying silk ligatures at the gingival margins of maxillary and mandibular bicuspids and molars. Subgingival bacterial samples were taken and periodontal destruction evaluated at baseline, and 3, 7 and 14 days after ligature placement. Dark-field microscopy was used to quantitate motile forms, spirochetes, straight and curved rods, filaments, cocci and fusiforms. Levels of connective tissue attachment, crestal alveolar bone and the volume of bone were assessed histometrically. Bacterial and histometric parameters were analyzed using analysis of variance. The results indicated that subgingival plaque, prior to induction of periodontitis, consisted primarily of cocci. At three days after ligature placement, cocci were significantly decreased, while straight rods became the predominant bacterial group for the remainder of the study. Spirochetes and motile forms were virtually absent at all time points. Histometric analyses showed significant loss of connective tissue attachment at three and seven days, which was reversed and repaired at 14 days. Factors relating to initiation, potentiation, and reversibility of connective tissue attachment loss are discussed.