Abstract
The sensitivity of erythrocyte glutamic-pyruvic transaminase (GPT) activity for assessment of vitamin B6 nutriture was demonstrated by a significant correlation between vitamin intake and GPT activity. The effects of dietary calorie, protein and amino acid intakes on vitamin B6 requirement were investigated in male rats using erythrocyte GPT activity as a criterion of vitamin B6 nutriture. There was no significant difference in erythrocyte GPT activity between hypothalamic-hyperphagic and control rats indicating that an excessive calorie intake does not alter apparent vitamin B6 requirement. With a 40-μg pyridoxine intake daily, there was a decrease in erythrocyte GPT activity as the level of protein in the diet increased, indicating that vitamin B6 requirement is apparently related to the level of dietary protein. Neither amino acid imbalance (8% casein + 15% gelatin) nor methionine toxicity (8% casein + 3% dl-methionine) appeared to alter vitamin B6 requirement as judged by erythrocyte GPT activity.
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