Abstract
Acetivibrio cellulolyticus and Clostridium thermocellum were unable to grow in medium containing 2 g litre −1 of untreated steam- and explosion-decompressed (SED) aspen wood as a source of carbon. When the medium was concurrently supplemented with 5 g litre −1 of Whatman CF11 cellulose powder, growth of both anaerobes, to levels comparable to media containing only CF11 cellulose, was observed. Treatment of the SED wood with ethanol-water, dioxane-water or sodium hydroxide released adhered lignin or lignin residues and rendered the SED wood suitable for the growth of both anaerobes. The results indicate that at concentrations of 4 g litre −1 or lower, growth on untreated SED wood is probably prevented because of inaccessibility of adherence sites for these bacteria, and at concentration of 4 g litre −1 or more it is also inhibited due to increased concentration of inhibitory materials such as lignin and lignin residues.
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