Abstract
Chloroplasts from spinach ( Spinacia oleracea L.) can be resolved by isopycnic centrifugation in density gradients of the silica sol Ludox AM into two zones, one containing the intact and the other the stripped Chloroplasts. Factors affecting the separation and the photosynthetic activity of the intact chloroplasts recovered from the gradients were investigated and a standard set of conditions proposed. The anomalous influence of polyethylene glycols on the banding densities of both stripped and intact chloroplasts was studied and the observed “density shifts” discussed in terms of their possible cause and significance. The general method was scaled up by means of continuous-flow zonal centrifugation to prepare intact chloroplasts in amounts corresponding to tens of milligrams of chlorophyll.
Published Version
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