Abstract
Temperature studies on nude mouse skin in vitro indicated that a complete deterioration of the barrier to urea and water permeation occurred after 48 h (irrespective of temperature), with the most pronounced effect at 50 ° C. The deterioration of the permeation barrier with increasing time of hydration was a very significant factor in the increasing permeation pattern of urea and water. This study indicated that the closed diffusion cell system is unsuitable as a method for determining percutaneous absorption in vitro. Because of the changes in the permeability characteristics that accompany deterioration of skin structure, the permeability data obtained with the closed diffusion cell system may be unrepresentative of normal skin. However, it may still have limited applications e.g. to determine the percutaneous absorption of highly toxic substances prior to in vivo tests, provided a few aspects are kept in mind, namely: experiments should not be run at temperatures above 37 ° C or longer than 24 h.
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