Abstract
Extracts from the venom sacs of the Oriental hornet ( V. orientalis) pupae are devoid of either direct or indirect hemolytic activity. However, in venom sac extract obtained during and following eclosion of the imagines, hemolytic activity of both types is present and gradually rises, reaching maximal levels at days 3 or 4. Complete daily evacuation of the venom sac for 5 consecutive days, results in progressive diminution of its hemolytic activity. Soybean trypsin inhibitor and, to a lesser degree, other protease inhibitors, partially suppress the direct hemolytic activity of V. orientalis venom, thus suggesting the possible role of venom proteases as potentiators of the direct hemolytic activity.
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