Abstract
IN EARLIER papers (van Overbeek, Conklin and Blakeslee, 1941, 1942) it was reported that Datura embryos, wheni isolated at the heart-shaped stage, require for growth the addition to the medium of some factor present in coconut milk. Later (van Overbeek, 1942) embryos isolated at the proembryo stage were also successfully cultivated in vitro by using fractionation products of coconut milk. The substance (or substances) responsible for the activity of coconut milk on the growth of embryos was called embryo factor (van Overbeek, 1942). Soon after it was found that coconut milk contained substances essential for the growth of embryos in vitro, investigations were started for the purpose of identifying the chemical composition of the embryo factor. In the course of this work, in which the growth of the embryos was used as a measure of embryo factor activitv, it became necessary to know more precisely the conditions which affect the growth of the embryos. The following points were investigated: (a) effect of temperature, (b) effect of pH, (c) effect of sucrose, (d) embryo factor activity of pure substances and extracts, (e) inhibitory substances, and (f) fractionation of coconut milk for embryo factor.
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