Factors Affecting the Expansion of Chondrocytes Harvested from Rabbit Auricle and Nasal Septum

Abstract

Received October 6, 2014 Revised November 25, 2014 Accepted November 26, 2014 Address for correspondence Jeong-Hoon Oh, MD, PhD Department of OtorhinolaryngologyHead and Neck Surgery, College of Medicine, The Catholic University of Korea, 180 Wangsan-ro, Dongdaemun-gu, Seoul 130-709, Korea Tel +82-2-958-2148 Fax +82-2-959-5375 E-mail ojhent@catholic.ac.kr Background and ObjectivesZZIn tissue engineering of the cartilage, cellular expansion is an essential step for creating useful amounts of tissue required. Many variables such as media composition, growth factors and cell seeding density have been utilized to stimulate the ability of cellular proliferation and to reduce the number of expansion in monolayer. The object of this study was to evaluate the effects of human serum (HS) and rabbit serum (RS) on cellular proliferation of auricular and nasal septal chondrocytes of the rabbit. Materials and MethodZZAuricular and nasal septal chondrocytes of the New Zealand White Rabbit were expanded in monolayer in medium supplemented with 2% RS, 5% RS, 10% RS, or 10% HS with or without adding of growth factors. The effect of the supplements on the cellular proliferation was compared. ResultsZZThe proliferation yield of chondrocytes grown in RS was comparable to that with HS. Both serums results in increased proliferation; however, the amount of cellular proliferation was not correlated with the concentration of the serum. The proliferation rate of nasal septal chondrocytes was higher than that of auricular chondrocytes. ConclusionZZBoth HS and RS resulted in the increased proliferation of rabbit auricular and nasal septal chondrocytes. The results show that the determination of appropriate types and concentrations of serum is required for cell expansion in constructing tissue-engineered cartilage. Korean J Otorhinolaryngol-Head Neck Surg 2015;58(2):95-100