Factors affecting the digestion of oesophageal fistula samples and hay samples in nylon bags in the rumen of cattle

Abstract

SUMMARYFactors affecting the nylon-bag technique, for examination of digestion in the rumen of extrusa and hay samples of low nutritional value, were studied. Effects of: the method of placement of bags in the rumen; saliva in extrusa; drying and milling techniques; a second-stage digestion with acid-pepsin solution; sample size; and losses of particulate matter through bag cloth were studied. Digestion of dry matter (d.m.) and cell walls after 12, 24, 48, 72 and 168 h in the rumen were used to indicate treatment effects.Attaching bags with nylon cord to a weight was a better method than enclosing them in perforated jars in the rumen which restricted digestion. Drying technique had little effect on digestion, although frozen extrusa was digested more than oven-dried feed, especially in samples of Heteropogon contortus. Chewing of forage was sufficient to obtain maximum rates of digestion. Milling extrusa through 1 or 2 mm screens did not increase digestion. An extra period of digestion in acid-pepsin solution increased digestion of feed samples more than that of extrusa, but the effects were small. It reduced between-bag variation slightly, but did not alter the significance of differences between treatments. The effect of sample size on digestion was small. Sample weight (3, 6 and 9 g) was kept proportional (42 mg/cm2) to bag cloth area.Digestion of d.m. after 24 h reflected digestion of cell walls. Digestion of cell walls was faster in grasses than in legumes. The higher digestibility of legumes was due to solubilization within 24 h of the greater amounts of cell contents found in legumes. Cell contents were only partially solubilized, and up to 10 g per 100 g original d.m. were not removed. Losses of particulate matter averaged 4·6% (range 0·5–10·6%). Losses were high when samples were finely milled (1 mm screen) but varied between species. Pore aperture of cloth was 25 μm. Methods for determining loss and correction of digestion data for such losses are given.