Abstract
The reassembly of cowpea chlorotic mottle virus, brome mosaic virus, and broad bean mottle virus was examined under a variety of conditions. The experimental basis is presented for optimal reassembly occurring in terms of infectivity and sedimentation behavior when protein in 1 M NaCl near neutrality and RNA are mixed stoichiometrically prior to dialysis at about 4° against near-neutral low ionic strength solutions containing at least 5 × 10 −3 M MgCl 2 or various polyamines. The consequences of omitting divalent cations, lowering pH levels, and varying the ratios of protein and RNA are described. The reassembly process is discussed in terms of the conditions investigated and related particularly to the properties of natural cowpea chlorotic mottle virus.
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